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TF20121201

Neofect DNA transfection reagent

目錄價(jià) ¥1500.00
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Neofect™ DNA transfection reagent

產(chǎn)品描述

Neofect™ DNA transfec on reagent 非脂質(zhì)陽(yáng)離子聚合物為主的轉(zhuǎn)染試劑,是新一代非病毒轉(zhuǎn)染試劑。

可以與 DNA 形成穩(wěn)定的復(fù)合物,透過(guò)細(xì)胞膜進(jìn)入細(xì)胞內(nèi),并保護(hù) DNA 免受核酸酶的降解。該試劑對(duì)細(xì)胞毒性
很小,可在含血清與抗生素的完全培養(yǎng)基中充分發(fā)揮作用。

低細(xì)胞毒性

Neofect™ DNA transfection reagent是一種非常溫和的轉(zhuǎn)染試劑,相比脂質(zhì)體轉(zhuǎn)染試劑,表現(xiàn)出更低的細(xì)胞毒性。在10倍使用濃度下,仍無(wú)明顯細(xì)胞毒性.

     
DNA-1

高轉(zhuǎn)染效率

Neofect™ DNA transfection reagent轉(zhuǎn)染效率與優(yōu)勢(shì)脂質(zhì)體轉(zhuǎn)染試劑LP2K相比,表現(xiàn)出更高的轉(zhuǎn)染效率。
DNA-2

性價(jià)比更高

使用Neofect™ DNA transfection reagent轉(zhuǎn)染細(xì)胞,非常方便只要簡(jiǎn)單的一比一等量混合
(1ug DNA :1ul Neofect™),即可實(shí)現(xiàn)完美轉(zhuǎn)染。相對(duì)與其它轉(zhuǎn)染試劑1:2或1:4的使用濃度,轉(zhuǎn)染試劑的使用量更少,價(jià)格更優(yōu)惠。同時(shí)更少的DNA的質(zhì)量,對(duì)細(xì)胞傷害更小。

ReagentCulture vesselVolume of reagentAmount of DNA
Neofect™ 6-well/35 mm dish 2μL 2μg
L2K 6-well/35 mm dish 10μL 4 µg

操作更方便

使用Neofect DNA transfection reagent 只需要簡(jiǎn)單的三步即可完成轉(zhuǎn)染實(shí)驗(yàn),一般情況下轉(zhuǎn)染后無(wú)需換液,即可實(shí)現(xiàn)高效轉(zhuǎn)染。

DNA-3

                

使用本產(chǎn)品發(fā)表的文章:

  • [1]. Sun L, Zhou R, Yang G, et al. Analysis of 138 pathogenic mutations in presenilin-1 on the in vitro production of Aβ42 and Aβ40 peptides by γ-secretase[J]. Proceedings of the National Academy of Sciences, 2016: 201618657.
  • [2]. Zhao X Q, Zhu L L, Chang Q, et al. C-type lectin receptor dectin-3 mediates trehalose 6, 6′-dimycolate (TDM)-induced Mincle expression through CARD9/Bcl10/MALT1-dependent nuclear factor (NF)-κB activation[J]. Journal of Biological Chemistry, 2014, 289(43): 30052-30062.
  • [3]. Gao X, Shi X, Fu X, et al. Human Tudor staphylococcal nuclease (Tudor‐SN) protein modulates the kinetics of AGTR1‐3′ UTR granule formation[J]. FEBS letters, 2014, 588(13): 2154-2161.
  • [4]. Luo Q, Liu X, Zheng Y, et al. Ephrin-B2 mediates trophoblast-dependent maternal spiral artery remodeling in first trimester[J]. Placenta, 2015, 36(5): 567-574.
  • [5]. Hao R, He J, Liu X, et al. Inhibition of hepatitis B virus gene expression and replication by hepatocyte nuclear factor 6[J]. Journal of virology, 2015, 89(8): 4345-4355.
  • [6]. Liu X, Hu Y, Liu X, et al. EPHB4, a down stream target of IFN‐γ/STAT1 signal pathway, regulates endothelial activation possibly contributing to the development of preeclampsia[J]. American Journal of Reproductive Immunology, 2016, 76(4): 307-317.
  • [7]. Li R, Li Y, Hu X, et al. Transcription factor 3 controls cell proliferation and migration in glioblastoma multiforme cell lines[J]. Biochemistry and Cell Biology, 2016, 94(3): 247-255.
  • [8]. Liu X, Hu Y, Zheng Y, et al. EPHB4 Regulates Human Trophoblast Cell Line HTR-8/SVneo Function: Implications for the Role of EPHB4 in Preeclampsia 1[J]. Biology of Reproduction, 2016, 95(3): 65, 1-11.
  • [9]. Wang L, Xu K, Ren C, et al. Targeted Deletion of Vitamin D receptor Gene in Mammalian Cells by CRISPR/Cas9 Systems[J]. bioRxiv, 2016: 040147.
  • [10]. Li P, Sun X, Ma Z, et al. Transcriptional Reactivation of OTX2, RX1 and SIX3 during Reprogramming Contributes to the Generation of RPE Cells from Human iPSCs[J]. International journal of biological sciences, 2016, 12(5): 505.
  • [11]. Liu X, Luo Q, Zheng Y, et al. NOTCH4 signaling controls EFNB2-induced endothelial progenitor cell dysfunction in preeclampsia[J]. Reproduction, 2016, 152(1): 47-55.
  • [12]. Zhang T, Yin Y, Liu H, et al. Generation of VDR Knock-Out Mice via Zygote Injection of CRISPR/Cas9 System[J]. PloS one, 2016, 11(9): e0163551.
  • [13]. Geng T, Huang Y, Hou C, et al. Inductive expression patterns of genes related to Toll signaling pathway in silkworm (Bombyx mori) upon Beauveria bassiana infection[J]. Journal of Asia-Pacific Entomology, 2016, 19(3): 861-868.
  • [14]. Xiong Y, Liu Y, Xiong W, et al. Hypoxia-inducible factor 1α-induced epithelial–mesenchymal transition of endometrial epithelial cells may contribute to the development of endometriosis[J]. Human Reproduction, 2016, 31(6): 1327-1338.
  • [15]. Liu H, Ren C, Zhu B, et al. High-Efficient Transfection of Human Embryonic Stem Cells by Single-Cell Plating and Starvation[J]. Stem cells and development, 2016, 25(6): 477-491.
  • [16]. Wang H, Zhang C, Xu L, et al. Bufalin suppresses hepatocellular carcinoma invasion and metastasis by targeting HIF-1α via the PI3K/AKT/mTOR pathway[J]. Oncotarget, 2016, 7(15): 20193-20208.
  • [17]. Wang Y, Liang D, Zhu Z, et al. Bone morphogenetic protein-7 prevented epithelial-mesenchymal transition in RLE-6TN cells[J]. Toxicology Research, 2016, 5(3): 931-937.
  • [18]. Geng T, Lv D D, Huang Y X, et al. JAK/STAT signaling pathway-mediated immune response in silkworm (Bombyx mori) challenged by Beauveria bassiana[J]. Gene, 2016, 595(1): 69-76.
  • [19]. Liu W, Liu X, Luo M, et al. dNK derived IFN-γ mediates VSMC migration and apoptosis via the induction of LncRNA MEG3: A role in uterovascular transformation[J]. Placenta, 2017, 50: 32-39.
  • [20]. Liu X, Luo Q, Zheng Y, et al. Notch1 Impairs Endothelial Progenitor Cell Bioactivity in Preeclampsia[J]. Reproductive Sciences, 2017, 24(1): 47-56.

注:本產(chǎn)品僅供研究使用,不可用于人或動(dòng)物的體外診斷與治療。
      For labortory use only. Not for diagmpstic or therapeutic use.

                

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